HMGA2 immunostaining is a straightforward technique which helps to distinguish pulmonary fat-forming lesions from normal adipose tissue in small biopsies: a retrospective observational study about a series of 13 lung biopsies

نویسندگان

  • Nicolas Piton
  • Émilie Angot
  • Florent Marguet
  • Jean-Christophe Sabourin
چکیده

BACKGROUND A tracheobronchial lesion observed during an endoscopic examination is usually sampled by the pulmonologist and sent to the pathologist for microscopic examination. Adipocytes may be observed in the lamina propria of tracheobronchial biopsies, which may complicate diagnosis of sampled lesions because these adipose cells may be part of the lesion (lipoma or pulmonary hamartoma), but may also be a normal component of the bronchial mucosa. Because endoscopic samples frequently miss their target, adipocytes observed in such biopsies usually lead to uncertainty regarding diagnosis. Both pulmonary hamartomas and lipomas have a high frequency of translocations involving HMGA2, resulting in over expression of the fusion protein. The literature suggests that only 31% of tracheobronchial lipomas are correctly diagnosed on biopsy, sometimes leading to unnecessary aggressive surgical resection. METHODS We performed retrospective study of tracheo-bronchial biopsies containing adipocytes using HMGA2 immunostaining in order to define their nature and to assess the diagnostic utility of this marker. RESULTS In total, 13 lesions biopsied in 12 patients and containing adipocytes were immunostained for HMGA2. Nuclear immunostaining was detected in 7 out of the 13 lesions (54%), allowing us to diagnose a lipoma or hamartoma. CONCLUSION HMGA2 immunostaining is an affordable and straightforward technique for accurate description of biopsies containing adipose cells. When positive, a diagnosis of benign adipose lesion can be made with confidence since well-differentiated liposarcomas have never been described in the tracheobronchial tree. Our work enabled us to diagnose a benign adipose lesion in 54% of cases, above the rate of 31% reported in the literature, based solely on morphological analysis. Overall, HMGA2 immunostaining could help pathologists to provide accurate diagnosis of tracheobronchial adipose lesions, leading to conservative treatment, for the overall benefit of patients.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The diagnostic value of TTF1, P63, HMWK [34βE12], CK7, and CD56 immunostaining in classification of lung carcinoma

Background & objective: The histologic distinction of small cell from non-small cell lung carcinoma and correct identification of all subtypes of lung carcinoma are very important in treatment management. The main method for histologic classification of lung tumors is based on morphology. However, in small bronchoscopic biopsies in par...

متن کامل

بررسی نمونه‌های آسیب‌شناسی حاصل از بیوپسی سوزنی با هدایت سی‌تی‌اسکن در ضایعات قفسه سینه بیماران مراجعه‌کننده به بخش پاتولوژی بیمارستان شهید صدوقی یزد

Introduction: Despite the popularity of needle biopsy for obtaining tissue samples in several solid organs, its use in thoracic lesions has been very restricted in some hospitals. This study intended to investigate the CT-Guided transthoracic core needle biopsy specimens in Yazd Shahid Sadoughi hospital during 2001- 2010. Methods: This is a retrospective cross- sectional study conducted on pat...

متن کامل

Expression of Apoptosis Related and Proliferative Proteins in Malignant Lympho-Proliferative Disorders

Background & objective: The current study aimed to perform an immunohistochemical analysis of patterns of apoptotic and cell proliferative related protein expression in different histological grades and immune phenotypes of malignant lymphomas and other lymphoproliferative disorders Methods:This observational study was carried o...

متن کامل

Positive nuclear BAP1 immunostaining helps differentiate non-small cell lung carcinomas from malignant mesothelioma

The differential diagnosis between pleural malignant mesothelioma (MM) and lung cancer is often challenging. Immunohistochemical (IHC) stains used to distinguish these malignancies include markers that are most often positive in MM and less frequently positive in carcinomas, and vice versa. However, in about 10-20% of the cases, the IHC results can be confusing and inconclusive, and novel marke...

متن کامل

Histopathological study of naturally occurring ovine pulmonary adenocarcinoma in native goat in Khuzestan, Iran

Sheep (ovine) pulmonary adenocarcinoma (SPA), also known as ovine pulmonary adneomatosis (OPA),jaagsiekte and ovine pulmonary carcinoma (OPC) is a contagious lung tumor in sheep and, more rarely, ingoats. This study was carried out in Khuzestan, in the southwest of Iran for a period of six months. A totalnumber of 3985 native breed goats were clinically inspected and postmortem examination was ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 12  شماره 

صفحات  -

تاریخ انتشار 2017